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Phosphatase inhibition assays as alternative for the detection of okadaic acid toxins

June 2011. ZEU-INMUNOTEC has recently attended to the XI Iberian Meeting on Harmful Algae and Biotoxins which took place in Bilbao, Spain. Dr Smienk and collaborators presented an in-house validation and ruggedness study of the ready-to-use phosphatase inhibition assay for the detection of Okadaic toxins. (Okatest, before known as Toxiline-DSP kit) Phosphatase inhibition assays could be used as an alternative for the detection of the okadaic acid toxins, as phosphatases are known to be their natural target. ZEU-INMUNOTEC has validated the commercially available Okatest kit (based on the phosphatase inhibition assay) according to international recognized guidelines for method validation (AOAC, EUROCHEM).

The repeatability was evaluated with 2 naturally contaminated samples. The relative standard deviation (RSD) calculated was 1.4 % at a level of 276 g/kg and 3.9 % at a level of 124 g/kg (n = 8). Intermediate precision was estimated by testing 10 different samples (mussel and scallop) on three different days and ranged between 2.4 and 9.5%. The IC50 values of the phosphatase used in this assay for OA, DTX-1 and DTX-2, together with accuracy and method comparison were also studied.

An in-house validation is only a first step to the complete a method validation. Okatest has also been validated by the European Union Reference Laboratory for Marine Biotoxins and a collaborative study with a total of 16 labs has been recently carried out. All the above studies have shown that the colorimetric phosphatase inhibition assay, Okatest, could be used as an alternative or complementary test for monitoring OA-toxins.

 

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Neogen acquires VeroMara from GlycoMar Ltd.

20-06-2011. Neogen Corporation (NASDAQ: NEOG) announced today that it has acquired the assets of the VeroMara seafood testing laboratory from its parent company, GlycoMar Ltd. Based in Oban, Scotland, VeroMara offers testing services to the shellfish and salmon aquaculture industries. VeroMara’s services include testing for shellfish toxins, general foodborne pathogens, including E. coli, noroviruses, and salmon husbandry. VeroMara recorded revenues of approximately $800,000 (U.S.) in its most recently completed fiscal year. Terms of the acquisition were not disclosed.


“The acquisition of VeroMara deepens Neogen’s advancement into the aquatic sciences,” said James Herbert, Neogen’s CEO and chairman. “VeroMara’s services are complementary and a nice fit with our existing product lines for the seafood industry, including one of our biggest food safety diagnostic products — our histamine tests for the tuna industry. The VeroMara purchase also provides Neogen with key collaborative relationships with influential aquaculture partners, and increased access to important international markets. “Our new operation in Oban will work in close cooperation with our Neogen Europe headquarters in Ayr, Scotland, providing expanded products and services to a larger customer base,” Herbert continued. “Our sales and marketing operations in Ayr have the capability to expand VeroMara’s business to several other European countries.” “The sale of our VeroMara testing service allows GlycoMar to focus on our core business — marine natural product research and development,” said Dr. Charles Bavington, GlycoMar’s managing director. “We are pleased to have VeroMara join Neogen, a company we respect as being one of the world leaders in food safety. This transition will guarantee uninterrupted quality service to our customers.”


In addition to its comprehensive line of rapid foodborne pathogen, sanitation monitoring and histamine tests that it offers the seafood industry, Neogen recently released rapid tests to detect toxins in shellfish that cause amnesic shellfish poisoning (ASP) and diarrhetic shellfish poisoning (DSP). The detection of ASP and DSP toxins has been a key service VeroMara has offered its customers. Neogen Corporation develops and markets products dedicated to food and animal safety. The company’s Food Safety Division markets dehydrated culture media, and diagnostic test kits to detect foodborne bacteria, natural toxins, genetic modifications, food allergens, drug residues, plant diseases and sanitation concerns. Neogen’s Animal Safety Division markets a complete line of diagnostics, veterinary instruments, veterinary pharmaceuticals, nutritional supplements, disinfectants, and rodenticides.

 

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