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Autochthon Biotechnology

Commercialisation Strategies for Functional Food, Feed and Biofuels from Algae

(C) Wolf G Kroner 2012April 2012.  In order to turn algal biomass into a commodity product current cost is to be significantly brought down. Dr James Flatt, CTO of Synthetic Genomics provides in-depth answers to probing questions by B2Bioworld about SGI’s R&D strategies, its business case, social responsibility and returns to investors – including Agradis and developing countries.

 

 

 

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Extraction of Nutraceutical Components from Algae Using Supercritical CO2

Nadine Igl-Schmid, Josef Schulmeyr, Andreas Wuzik, Technical article peer reviewed & revised by authors of NATECO2 (Joh. Barth & Sohn)

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Phosphatase inhibition assays as alternative for the detection of okadaic acid toxins

June 2011. ZEU-INMUNOTEC has recently attended to the XI Iberian Meeting on Harmful Algae and Biotoxins which took place in Bilbao, Spain. Dr Smienk and collaborators presented an in-house validation and ruggedness study of the ready-to-use phosphatase inhibition assay for the detection of Okadaic toxins. (Okatest, before known as Toxiline-DSP kit) Phosphatase inhibition assays could be used as an alternative for the detection of the okadaic acid toxins, as phosphatases are known to be their natural target. ZEU-INMUNOTEC has validated the commercially available Okatest kit (based on the phosphatase inhibition assay) according to international recognized guidelines for method validation (AOAC, EUROCHEM).

The repeatability was evaluated with 2 naturally contaminated samples. The relative standard deviation (RSD) calculated was 1.4 % at a level of 276 g/kg and 3.9 % at a level of 124 g/kg (n = 8). Intermediate precision was estimated by testing 10 different samples (mussel and scallop) on three different days and ranged between 2.4 and 9.5%. The IC50 values of the phosphatase used in this assay for OA, DTX-1 and DTX-2, together with accuracy and method comparison were also studied.

An in-house validation is only a first step to the complete a method validation. Okatest has also been validated by the European Union Reference Laboratory for Marine Biotoxins and a collaborative study with a total of 16 labs has been recently carried out. All the above studies have shown that the colorimetric phosphatase inhibition assay, Okatest, could be used as an alternative or complementary test for monitoring OA-toxins.

 

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life'sDHA™ Adds Tremendous Marketing Value - DHA for Dietary Supplements

Docosahexaenoic acid, DHA, is a long-chain omega-3 fatty acid that provides health benefits for people of all ages. DHA omega-3 is found throughout the body. Specifically, it is a major structural fat in the brain and retina accounting for up to 97% of the omega-3 fats in the brain and up to 93% of the omega-3 fats in the retina. DHA is also a key component of the heart. While it has been shown that DHA is important for health and development, it’s difficult for most people to get enough through diet alone. This is because the primary dietary sources of DHA are limited to organ meat and fatty fish. And, although expert bodies such as the American Heart Association and the USDA (through its Dietary Guidelines for Americans) recommend eating fish regularly, other agencies including the Food and Drug Administration (FDA) and Environmental Protection Agency (EPA) caution against eating certain types of fish because of contaminant concerns.
Fortunately, as the interest in improving health through nutrition continues to grow, and research continues to demonstrate just how important DHA is to our health, the demand for DHA supplements and foods fortified with DHA will continue to increase. Now is the time to capitalize on this DHA marketing opportunity. Whether you are looking to enhance a custom blend or differentiate yourself in the DHA omega-3 market, life'sDHA™ from algae is the smart choice.
Customers respond to high quality ingredients, such as life'sDHA™.
·    life'sDHA™, produced from algae, is a vegetarian source of DHA compatible with many foods or beverages. Algae is the only vegetarian source that provides a meaningful amount of DHA. Other vegetarian sources of omega-3s, such as flax seeds, walnuts and certain vegetable oils, contain ALA (alpha-linolenic acid), not DHA. As a vegetarian source of DHA, life'sDHA™ is suitable for use in traditionally vegetarian foods and beverages, such as breads, cereals, yogurts, cheeses, milks and juices. In addition, life'sDHA™ is both Kosher and Halal.
·    DHA is the only omega-3 shown to provide brain, eye and heart health benefits. EPA, found in fish oil, has been shown to support cardiovascular health but has not been proven to play a role in brain or eye health. ALA, found in flaxseed oil, has no known independent benefits on brain or eye health and there are insufficient data to support its role in cardiovascular health.
·    life'sDHA™ is produced in a controlled environment, eliminating the risk of oceanic contamination. From start to finish, life'sDHA™ is produced in an FDA-inspected facility, under current Good Manufacturing Practice (cGMP) regulations with controls in place to ensure the highest quality product. Because the algae is grown outside of the ocean, there is no risk of ocean-borne contaminants such as PCBs and dioxins.
·    In March of 2004, the U.S. Food and Drug Administration (FDA) completed a favorable review of the Company's generally recognized as safe (GRAS) notification regarding the use of its DHA oil as an ingredient in foods and beverages.
·    life'sDHA™ also has Novel Foods approval for use in supplements and a variety of foods in Europe as well as Australia/New Zealand.
·    life'sDHA™ is a high quality product with excellent sensory characteristics and shelf life. Made from a patented vegetarian source and production process, life'sDHA™ will not adversely affect your product’s sensory characteristics. In addition, life'sDHA™ is highly concentrated so less is needed. Foods and beverages fortified with life'sDHA™, including cereal bars, yogurts, bread, cheese and soymilk, have demonstrated excellent shelf life and taste performance.
www.martek.com

Cognis: Betatene natural mixed carotenoids

Betatene® natural mixed carotenoids contain several carotenoids derived from Dunaliella salina algae that are not only popular with consumers, but also well investigated by the scientific community. Betatene® provides a unique combination of important carotenoids that are found in many fruits and vegetables.
• Betatene® contains beta-carotene, alpha-carotene, lutein, zeaxanthin, and cryptoxanthin.
• Betatene® supplies several natural isomers (cis and trans) of beta-carotene, unlike the synthetic products, which contain mainly one isomer (all trans).
• The microscopic alga Dunaliella salina is cultivated by Cognis Australia in salt water from the clean and remote Southern and Indian Oceans of Australia.
• The two sites at Hutt Lagoon,Western Australia and Whyalla, South Australia are the world’s largest algae farms with a total capacity of over 1,900 acres (800 hectares).Two sites give security of supply and year round production.
• Innovative, proprietary techniques and rigorous scientific monitoring maximize the growth of these carotenoid-rich algae in a sustainable fashion with positive environmental impact.
• The algae are harvested mechanically, without any chemical additives or solvents.The carotenoids are then extracted and formulated under Cognis total process control using
only natural plant oils.
• Betatene® can be labeled as natural mixed carotenoids (carotenes), natural beta-carotene, algae carotenes, or as provitamin A* (because the human body converts beta-carotene and
alpha-carotene to vitamin A).
• Betatene® is available in multiple concentrations and forms (oil, powder, and tablet-grade beadlets) to meet customer formulation requirements.
• Betatene® has excellent stability. Stability charts illustrate Betatene® products maintain their potency and stability over time. In fact, only a minimal overage is required in most formulations.


The typical carotenoid distribution in
Betatene® 30% is as follows:


                       microgram/gram
beta-carotene      283,500
alpha-carotene       10,500
cryptoxanthin          3,000
lutein                     1,500
zeaxanthin              1,500


www.cognis.com

*depending upon local regulations.

Extraction of Nutraceutical Components from Algae Using Supercritical CO2

Nadine Igl-Schmid, Josef Schulmeyr, Andreas Wuzik

Supercritical CO2 extraction pressuresDuring food processing organic or inorganic liquids are conventionally used for separation purposes. While supercritical CO2 extraction is increasingly applied as an alternative at industrial scale, details of processing  are critically important to preserve functional properties of the ingredients, for example carotenoids in nutritional products or dietary supplements. The authors describe algae species which they find best suited for carotenoid extraction and their experience with extraction from sensitive biomass at different pressures with varying outcomes of  supercritical CO2 extraction.

 

Authors work with brewing and nutrition industries supplier Barth-Haas Group

 

A timely, peer-reviewed professional overview within B2Bioworld’s Algae Series.

 

Citation Reference:

Igl-Schmid N, Schulmeyr J, Wuzik A (2011): Extraction of Nutraceutical Components from Algae using Supercritical CO2. Published online,  March 1. B2Bioworld.com

 

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Harboes Brewery places a bet on microbial enzymes and eco-emotions



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