Enzymatic Hydrolysis of Corn Stover: Improved pre-treatment with Trametes Hirsuta yj9

Chinese Academy of Sciences Chengdu Institute of Biology

·    Trametes hirsuta yj9 preferentially degrade lignin over cellulose
·    Laccase is the major ligninolytic enzyme
·    Sugar yields of pretreated corn stover were significantly increased
·    The structure of pretreated corn stover showed significant changes
·    Sugar yields were inversely proportional to the lignin contents.

17-01-2012. Corn stover, mainly composed of lignin, cellulose and hemicellulose, is one of the most abundant agricultural byproducts in China. Approximately 200 million tons of corn stover is produced in China per year. Most of the corn stover is burned or directly discarded resulting in resource waste and environment pollution. Lignocellulose can be enzymatically hydrolyzed and transformed into important biofuels such as ethanol and butanol. The process can transform 30% energy and reduce environmental pollution. So in their study, Prof. LIU Xiaofeng's team from CAS Chengdu Institute of Biology isolated a newly Trametes hirsuta yj9 to pretreat corn stover in order to enhance enzymatic digestibility.

They demonstrated that T. hirsuta yj9 preferentially degraded lignin to be as high as 71.49% after 42-day pretreatment. Laccase and xylanase was the major ligninolytic and hydrolytic enzyme, respectively and filter paper activity (FPA) increased gradually with prolonged pretreatment time. Meanwhile, researchers found that sugar yields increased significantly after pretreatment with T. hirsuta yj9, reaching an enzymatic digestibility of 73.99% after 42 days of pretreatment. Scanning electron microscopy (SEM) showed significant structural changes in pretreated corn stover, the surface of pretreated corn stover became increasingly coarse, the gaps between cellulose fibers were visible, and many pores were developed.

Researchers also demonstrated correlation analysis showed that sugar yields were inversely proportional to the lignin contents, less related to cellulose and hemicellulose contents. LIU’s research got supports from Chinese Academy of Science, National High Technology Research and Development Program of China.
Source: Chinese Academy of Sciences

References
Feng-hui Sun FH, Li J, Yuan YX, Yan ZY, Liu XF (2011): Effect of biological pretreatment with Trametes hirsuta yj9 on enzymatic hydrolysis of corn stover. International Biodeterioration and Biodegradation: October: 931-938.

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03-01-2012. Philadelphia (Pennsylvania ) and Ludwigshafen (Germany). BASF is participating with $ 30 million through BASF Biorenewable Beteiligungs-GmbH & CoKG in the American technology firm Renmatix Inc. The BASF subsidiary led a $50 million financing round, joined by new and existing investors.

The technology company Renmatix has developed the Plantrose™ platform. With this patented process, industrial sugar can be produced from lignocellulosic biomass (wood, cane trash or straw). This technology makes it possible for the first time to produce industrial sugar in large quantities and at competitive cost from non-edible plant mass. “The Plantrose technology could allow us in the future to broaden our use of renewable raw materials while improving the cost effectiveness of our value chains even further. In the partnership with Renmatix, BASF is pursuing a new direction while simultaneously underlining its corporate strategy of offering even more sustainable solutions,” said Dr. Josef R. Wünsch, Senior Vice President Modelling, Formulation Research and Technology Incubation at BASF.

In the Plantrose technology, biomass is split into cellulose and sugar in supercritical water at high temperature and pressure in a two-step process. Since the Plantrose technology utilizes non-edible biomass as feedstock, it is not in competition with feed and food production. “Thanks to the partnership with BASF we can now develop and commercialize our technology more efficient. We have already demonstrated the functionality of the Plantrose process in a pilot plant. In cooperation with BASF, we will be moving it to the industrial scale,” said Mike Hamilton, Chief Executive Officer at Renmatix.

Industrial sugars are important renewable resources for the chemical industry and can be used, for example, to produce biofuels or basic chemical products and intermediates by fermentative processes. The availability of industrial sugars in sufficient quantities and at favorable cost is therefore important for the competitiveness of the products.

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