REALATED ARTICLES

Click on a word or phrase in our Tag Cloud and see all related articles listed below.

 

Current tag:

Phosphatase inhibition assays as alternative for the detection of okadaic acid toxins

June 2011. ZEU-INMUNOTEC has recently attended to the XI Iberian Meeting on Harmful Algae and Biotoxins which took place in Bilbao, Spain. Dr Smienk and collaborators presented an in-house validation and ruggedness study of the ready-to-use phosphatase inhibition assay for the detection of Okadaic toxins. (Okatest, before known as Toxiline-DSP kit) Phosphatase inhibition assays could be used as an alternative for the detection of the okadaic acid toxins, as phosphatases are known to be their natural target. ZEU-INMUNOTEC has validated the commercially available Okatest kit (based on the phosphatase inhibition assay) according to international recognized guidelines for method validation (AOAC, EUROCHEM).

The repeatability was evaluated with 2 naturally contaminated samples. The relative standard deviation (RSD) calculated was 1.4 % at a level of 276 g/kg and 3.9 % at a level of 124 g/kg (n = 8). Intermediate precision was estimated by testing 10 different samples (mussel and scallop) on three different days and ranged between 2.4 and 9.5%. The IC50 values of the phosphatase used in this assay for OA, DTX-1 and DTX-2, together with accuracy and method comparison were also studied.

An in-house validation is only a first step to the complete a method validation. Okatest has also been validated by the European Union Reference Laboratory for Marine Biotoxins and a collaborative study with a total of 16 labs has been recently carried out. All the above studies have shown that the colorimetric phosphatase inhibition assay, Okatest, could be used as an alternative or complementary test for monitoring OA-toxins.

 

__________________

Disclaimer: You agree that  B2Bioworld is not responsible and will not be held liable for any third party content on its sites or any third-party content, products or services available on other web sites accessed through links from B2Bioworld sites. Links to third-party sites are for your convenience only, and their inclusion on   B2Bioworld's sites does not imply any endorsement, guarantee, warranty or representation by  B2Bioworld.

Top 10 Tags

All Tags (2094)